Composite
Pc-PQSR

Part:BBa_K1157019:Design

Designed by: Lin Ang Chieh   Group: iGEM13_NTU-Taida   (2013-09-15)


Pc-PQSR


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 525
    Illegal BamHI site found at 141
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 752
    Illegal NgoMIV site found at 973
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 709


Design Notes

There are no restriction sites inside the sequence.


Source

PQSR is a pcr product of Pseudomonas aeruginosa ATCC 27853. The rest is provided by iGEM official biobrick.

References